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Arkistoitu opetusohjelma 2015–2016
Selaat vanhentunutta opetusohjelmaa. Voimassa olevan opetusohjelman löydät täältä.
KEMI4140 Field course in natural compound chemistry 5 ECTS
Periods
Period I Period II Period III Period IV
Language of instruction
English
Type or level of studies
Advanced studies
Course unit descriptions in the curriculum
Kemia
Department of Chemistry

Learning outcomes

The course aims at teaching the student how to detect the presence of cuticular and vacuolar polyphenolic metabolites together with polyphenols with high protein affinity from any given plant sample in non-optimal field conditions. At the same time the student learns how results of more simplified methods can guide the sample analysis by more sophisticated methods such as UPLC-DAD-MS/MS. The ultimate goal is to learn to rapidly screen plant species and organs to find polyphenols with certain chemical properties and to further characterize these polyphenols from the species or organs that were earlier screened positive.

General description

The course is divided into two sections. The first section combines the collection and analysis of exotic plant samples from the glasshouses of the Botanical Garden of the University of Turku. Each student samples several species of his/her own priority and rapidly screens the samples for their protein binding activities. The positive species will be further collected and preserved by different techniques (e.g. liquid nitrogen, freeze-drying, oven-drying) and analysed for their polyphenol composition by both the fingerprinting tools of UPLC-DAD connected to the triple quadrupole mass spectrometer and by the accurate mass measurement tools of UPLC-DAD connected to the Orbitrap mass spectrometer. The second section focuses on making the distinction between cuticular and vacuolar polyphenols with a freely selected set of tree species in the Botanical Garden of the University of Turku. The distiction will be first made by simplified tools in field conditions and the positive results will be verified by UPLC-DAD-MS/MS instruments, and the function of new chemotaxonomic tools will be tested. Depending on the year, small-scale insect herbivore assays may be included as well.

Teacher responsible

Juha-Pekka Salminen

Teaching

1-Mar-2016 – 31-May-2016

Evaluation

Numeric 0-5.

Evaluation criteria

Active participation and analysis of the samples is compulsory. Final report, no exam.

Further information

The first 2-3 day section will be organized in March (exact dates will be agreed with the students), and the second 2-3 day section in May (exact dates depending on Mother Nature). Both sections are run at the Botanical Garden of the University of Turku and the samples are further analysed at the lab of Natural Chemistry Research Group at the department of chemistry. Samples of the first section must be analysed during March-May 2016 and those of the second section during May-December 2016.

Registration during February 2016 via NettiOpsu.