Uusi opinto-opas (sisältäen myös opetusohjelmat) lukuvuodelle 2018-2019 sijaitsee osoitteessa https://opas.peppi.utu.fi . Tältä sivustolta löytyvät enää vanhat opinto-oppaat ja opetusohjelmat.

The new study guide (incl. teaching schedules) for academic year 2018-2019 can be found at https://studyguide.utu.fi. This site contains only previous years' guides.

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Arkistoitu opetussuunnitelma 2016–2018
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Opinto-oppaat » Matemaattis-luonnontieteellinen tiedekunta » Kemian laitos » Chemistry of Drug Development
KEMI6410 Preparative Chromatography and MS/MS Quantitation Methods 5 op
Organised by
Person in charge
Juha-Pekka Salminen
Preceding studies
BSc course in Natural Compound Chemistry is recommended

Learning outcomes

The course aims at teaching the student how different compounds are purified by preparative and semi-preparative HPLC both in theory and practise. The student also learns how to optimize and create a quantitative UPLC-MS/MS method for any compound that is ionizable via electrospray ionisation. At the same time the student learns to understand how MS/MS technology can be used to benefit structure characterization of unknown compounds.


The course deals with methods for large-scale (>100 mg) isolation and purification of different compounds and uses preparative and semi-preparative HPLC methods also in practise. Purified compounds are used to create and optimize a quantitative UPLC-DAD-MS/MS method. The developed method will be used to find limits of detection, limits of quantification and linear range for quantification for various analytes by (1) diode array, (2) full scan, (3) SIM (selected ion monitoring) as well as (4) MRM (multiple reaction monitoring) quantitation techniques.

Teaching methods

Teaching method Contact Online
Lectures 20 h 0 h
Exercises 40 h 0 h

Teaching language


Modes of study

Option 1
Available for:
  • Degree Programme Students
  • Other Students
  • Doctoral Students
  • Exchange Students
  • In English

Final report. No exam.


Numeric 0-5.

Recommended year of study

4. year autumn
5. year autumn

4th or 5th year, autumn 2016, period 1.

Course is held every second year. Course can have a maximum of 16 students. Registration 2 weeks before the course in Nettiopsu or by e-mail (j-p.salminen@utu.fi).

Study materials

Lecture notes together with material available in Moodle.

Belongs to following study modules

Archived Teaching Schedule. Please refer to current Teaching Shedule.
Implementation details are unavailable.
Kemian laitos
MDP in Materials Science