Uusi opinto-opas (sisältäen myös opetusohjelmat) lukuvuodelle 2018-2019 sijaitsee osoitteessa https://opas.peppi.utu.fi . Tältä sivustolta löytyvät enää vanhat opinto-oppaat ja opetusohjelmat.

The new study guide (incl. teaching schedules) for academic year 2018-2019 can be found at https://studyguide.utu.fi. This site contains only previous years' guides.

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Archived Curricula Guide 2016–2018
Curricula Guide is archieved. Please refer to current Curricula Guides
KEMI6416 Screening of Plants for Bioactivity and Bioactive Compounds 5 ECTS
Organised by
Chemistry
Person in charge
Juha-Pekka Salminen
Preceding studies
BSc course in Natural Compound Chemistry and MSc course in Chemistry of Tannins and Other Polyphenols are recommended

Learning outcomes

The course aims at teaching the student how to detect the presence of compounds with high protein affinity or high oxidative activity from any given plant sample by rapid small-scale tests. At the same time the student learns how results of more simplified methods can guide the sample analysis by more sophisticated methods such as UPLC-DAD-MS/MS. The ultimate goal is to learn to rapidly screen plant species and organs to find specific compound classes and compounds with certain bioactivities and to further characterize and quantify these from the species or organs that were earlier screened positive.

Contents

The course starts by the collection and analysis of exotic plant samples from the glasshouses of the Botanical Garden of the University of Turku. Each student samples several species of his/her own priority and rapidly screens the samples for their protein binding and oxidative activities. The positive species will be further collected for quantitative studies and preserved by different techniques (e.g. liquid nitrogen, freeze-drying, oven-drying). Each species will be separately subjected to enzymatic and pH 10 oxidation and for removal of compounds with high protein affinity. All bioactive compound classes and individual compounds will be revealed by quantitative polyphenol analysis with the MS/MS fingerprinting tools developed for UPLC-DAD-MS/MS.

Teaching methods

Lab practicals and joint meetings

Teaching language

English

Modes of study

Option 1
Available for:
  • Degree Programme Students
  • Other Students
  • Doctoral Students
  • Exchange Students
Participation in classroom work
  • In English
Essay
  • In English

Active participation in the practicals held in the Botanical Garden and the Department of Chemistry are compulsory. Supervised practicals are accompanied by more independent analysis of plant samples and results interpretation, followed by joint meetings with PowerPoint presentations to reveal the main results. Literature survey on the known chemical composition of the chosen bioactive plant species is included in the written final report. No exam.

Evaluation

Numeric 0-5.

Recommended year of study

4. year spring
5. year spring

4th or 5th year, spring 2018, period 4.
Course is held every second year. Course can have a maximum of 16 students. Registration 2 weeks before the course in Nettiopsu or by e-mail (j-p.salminen@utu.fi).

Study materials

Material available in Moodle

Belongs to following study modules

Department of Chemistry
2017–2018
Teaching
Archived Teaching Schedule. Please refer to current Teaching Shedule.
Implementation details are unavailable.
Department of Chemistry
DP in Chemistry
DP in Chemistry
Finnish Study Modules
MDP in Materials Science