Uusi opinto-opas (sisältäen myös opetusohjelmat) lukuvuodelle 2018-2019 sijaitsee osoitteessa https://opas.peppi.utu.fi . Tältä sivustolta löytyvät enää vanhat opinto-oppaat ja opetusohjelmat.

The new study guide (incl. teaching schedules) for academic year 2018-2019 can be found at https://studyguide.utu.fi. This site contains only previous years' guides.

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Archived teaching schedules 2016–2017
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KEMI6410 Preparative Chromatography and MS/MS Quantitation Methods 5 ECTS
Period I Period II Period III Period IV
Language of instruction
Type or level of studies
Advanced studies
Course unit descriptions in the curriculum
Department of Chemistry

Learning outcomes

The course aims at teaching the student how different compounds are purified by preparative and semi-preparative HPLC both in theory and practise. The student also learns how to optimize and create a quantitative UPLC-MS/MS method for any compound that is ionizable via electrospray ionisation. At the same time the student learns to understand how MS/MS technology can be used to benefit structure characterization of unknown compounds.

General description

The course deals with methods for large-scale (>100 mg) isolation and purification of different compounds and uses preparative and semi-preparative HPLC methods also in practise. Purified compounds are used to create and optimize a quantitative UPLC-DAD-MS/MS method. The developed method will be used to find limits of detection, limits of quantification and linear range for quantification for various analytes by (1) diode array, (2) full scan, (3) SIM (selected ion monitoring) as well as (4) MRM (multiple reaction monitoring) quantitation techniques.

Teacher responsible

Juha-Pekka Salminen


7-Sep-2016 – 19-Dec-2016
Lectures 20 h
Wed 7-Sep-2016 at 12-14, SEM 1, ARC 2.krs.
Fri 9-Sep-2016 - 7-Oct-2016 weekly at 8-10, SEM 1, ARC 2. floor
Tue 13-Sep-2016 at 8-10, SEM 1, ARC 2.krs.
Tue 27-Sep-2016 at 8-10, SEM 1, ARC 2. floor
Wed 28-Sep-2016 at 12-14, SEM 1, ARC 2. floor
Tue 4-Oct-2016 at 8-10, SEM 1, ARC 2.krs.
Mon 12-Dec-2016 at 10-14, SEM 1, Arcanum 2nd floor
Thu 15-Dec-2016 at 10-14, SEM 1, Arcanum 2nd floor
Mon 19-Dec-2016 at 14-16, SEM 1, Arcanum 2nd floor
Exercises 40 h


Numeric 0-5.

Further information

Course can have a maximum of 16 students.